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Membranes were blocked using 5 non fat milk in Tris buffered saline supplemented with 0 <a href=http://vlasixs.cyou>lasix kopen</a> Samples of 70 ОјL were then taken from the apical or basolateral chamber at predetermined time intervals 30, 60, 120, and 180 minutes and immediately replaced with the same volume of HBSS